KMID : 1011420130180040533
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Journal of Korean Ophthalmic Optics Society 2013 Volume.18 No. 4 p.533 ~ p.540
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Synaptic Pattern of NMDA R1 upon the Direction-Selective Retinal Ganglion Cells in Developing Mouse Retina
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Lee Jee-Geon
Kwon Oh-Ju Jeon Chang-Jin
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Abstract
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Purpose:To investigate the synaptic pattern of NMDA glutamate receptor subtype NMDA R1 on the dendritic
arbors of ON-OFF direction-selective retinal ganglion cells (DS-RGSs) in developing [(5,10) days postnatal (PN)]
mouse retina.
Methods:ON-OFF DS-RGCs were injected with Lucifer yellow and the cells were identified by
their characteristic morphology. To identify glutamatergic excitatory input from bipolar cell, we used a marker for
the membrane traffic motor protein kinesin.
Results:We identified DS-RGCs in P5, and P10 mouse retina. The
immunofluorescence labeling of NMDA R1 was most prominent in the IPL. Our results showed that their
presence upon the entire dendritic arbor of ON-OFF DS-RGCs is without any evidence of asymmetry, which
would predict direction selectivity.
Conclusions:The glutamatergic input from bipolar cell reveals symmetry
pattern in all periods of P5, and P10. The results may suggest that direction selectivity not lies in the specific
pattern of NMDA R1 receptors.
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KEYWORD
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Direction selectivity , NMDA receptor , Synaptic pattern , Retinal ganglion cell , Single cell injection , Immunocytochemstry
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